WebBioAssay record AID 1767130 submitted by ChEMBL: Antiproliferative activity against human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay. Web7月17日收录于话题ZetaLifeAdvancedDNARNA，AD600150转染试剂；成功转染THP-1(人单核白血病细胞)悬浮细胞；来自广州中山大学附属**医院客户的喜讯;出色的细胞转染结果与您分享;24小时荧光图片:zetalife，AdvancedDNARNA转染试剂信息如下 ...

FAQ: THP1 cell lines InvivoGen

WebJun 21, 2024 · 2.6 Cell counting kit-8 (CCK8) assay Transfected cells were adjusted to 3 × 10 4 /ml and plated into 96-well plates (3000 cells/well in 100 µL medium). After culturing for 24, 48, 72, and 96 h, the supernatant was replaced with 100 µL of serum-free medium added with 10 µL of CCK8 (Dojindo, Japan) and incubated at 37°C in the dark for 1 h. WebCCK-8 tests of (a) THP-1 cells and (b) THP-1-derived macrophages were assayed on Day 1, 3 and 6 in response to different Mg 2þ concentrations or RPMI 1640. *P < 0.05 vs. mono-ctr or m-ctr... tourismusverband murtal neu

The cell viability of THP-1 macrophages determined by

WebThe inflammatory stimulation assay was carried out based on Zhao’s method with slight modifications. 33 THP-1 macrophages were inoculated in a 96-well microplate with 1 × 10 5 /mL cell density and cultivated until cells adhered to the wall. Then cells were pretreated with CFP (100, 200, 300 μg/mL), stimulated by LPS (1.0 μg/mL) and hatched ... WebDay 1 1- Add 180 µl of THP1 cell suspension per well of a 96-well plate (~ 100 000 cells/well). 2- Treat THP1 cells with 20 µl of PMA (final concentration 20 - 50 ng/ml) for 3 … WebBioAssay record AID 1767130 submitted by ChEMBL: Antiproliferative activity against human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 … tourismusverband miesbach