WebSep 21, 2024 · In a basic form, PREM can be used to study the cytoskeleton architecture in a cell population. In an advanced form, it can be combined with immunogold staining to detect specific proteins in the cytoskeleton (Fig. 1), with light microscopy to correlate the cytoskeleton organization with cell behavior or with the distribution and dynamics of … WebApr 10, 2024 · In the model organism insect Drosophila melanogaster short cilia assemble on spermatocytes that elaborate into 1.8 mm long flagella during spermatid differentiation. A unique feature of these cilia/flagella is their lack of dependence on intraflagellar transport (IFT) for their assembly.
INDIVIDUAL MICROTUBULES VIEWED BY …
WebApr 1, 2024 · Growth cone architecture remains poorly understood at nanoscales. We therefore investigated mouse hippocampal neuron growth cones using cryo-electron tomography to directly visualise their three-dimensional subcellular architecture with molecular detail. Our data showed that the hexagonal arrays of actin bundles that form … WebNov 15, 2024 · The primary cilium has recently become the focus of intensive investigations into understanding the physical structure and processes of eukaryotic cells. This paper describes two tilt-series image datasets, acquired by transmission electron microscopy, of in situ chick-embryo sternal-cartilage primary cilia. These data have been released under … tsabotlhe
4.17: The Cytoskeleton - Intermediate Filaments and Microtubules
WebStudent Scholar Awardees. Zhaslan Baraissov — Cornell University. Measuring three-dimensional strain in Nb3Sn grains by combining ZOLZ and HOLZ diffraction. Samantha Berry — Pennsylvania State University. Diving into COVID-19: Visualizing SARS-CoV-2 Patient Proteins using Liquid-Electron Microscopy. Yutong Bi — University of California ... WebSpecialized cell junctions occur at points of cell-cell and cell-matrix contact in all tissues, and they are particularly plentiful in epithelia. Cell junctions are best visualized using either conventional or freeze-fracture electron … WebBecause of the nanometer-scale thickness of cytoskeletal fibers, electron microscopy (EM) is a key tool to determine the structure of the cytoskeleton. This article describes application of rotary shadowing (or metal replica) EM for visualization of the cytoskeleton. The procedure is applicable to thin cultured cells growing on glass coverslips ... phillis wheatley primary sources